| PRODUCT NAME: | Micro-Albumin |
| INTENDED USE: | Micro-Albumin is a competitive ELISA test system for the quantitative measurement of human albumin in urine. |
| PRODUCT OVERVIEW: | Proteins passing the glomerular basal membrane of the kidney undergo differentiated filtering. The permeability is inversely proportional to the molecular weight (albumin about 0.6%, myoglobulin about 75%). Nevertheless, only minimal quantities of protein are detectable in urine, because big quantities of protein are reabsorbed by the tubuli. Elevated glomerular protein permeability and high tubular plasma protein elimination can be differentiated by measuring the molecular weight distribution of the eliminated proteins. In case of a very active glomerular filtering process the albumin secretion can arise without an underlying kidney disease. This situation is called "microalbuminuria". In diabetes, albumin secretion is a very important parameter for the evaluation of the kidney function. Urine values higher than 25 µg/ml indicate a detrimental kidney function of insulin dependent (type I) and non-insulin dependent (type II) diabetic patients. The determination of albumin is therefore an important diagnostic tool in diabetic nephropathies. |
TECHNICAL SPECIFICATIONS:
|
| Method: | Enzyme Linked Immune Reaction |
| Capture: | Albumin |
| Calibrators: | 0.15, 1.5 , 6, 25, 100, 400 µg/ml |
| Sample type: | Serum or plasma |
| Sample volume: | 20 µl |
| Measuring range: | 1.5 - 400 µg/ml |
| Cut-off: | 25 µg/ml
|
| Reading: | Absorbance at 450 nm |
| Total time: | 50 minutes
|
| Storage: | 2 - 8 ºC
|
| Kit Contents: | Microplate
Calibrators: A, B, C, D, E, F
Controls: Positive & Negative
Enzyme Conjugate
TMB Substrate
Diluent
Stop Solution
Wash Concentrate
|