| PRODUCT NAME: | Anti-C1q |
| INTENDED USE: | Anti-C1q is an ELISA test for the quantitative determination of IgG antibodies against C1q complement factor in human serum or plasma. |
| PRODUCT OVERVIEW: | C1q is the first protein in the activation cascade of the classical complement pathway. Antibodies that bind to the collagen portion of C1q with high affinity have been detected in patients with a number of rheumatic disorders. In particular, Anti-C1q antibodies have been found in patients with systemic lupus erythematosus (SLE) and patients with hypocomplementemic urticarial vasculitis syndrome (HUVS). For SLE patients there exists an unambiguous correlation between antibody titer, disease progression and kidney involvement. SLE patients with an increasing Anti-C1q titer were observed to have an approximately 50% higher risk of developing lupus nephritis. C1q antibodies often appear a few weeks, sometimes as much as half a year before onset of nephritis. Continuous monitoring of the antibody status with Anti-C1q test is thus recommended. |
TECHNICAL SPECIFICATIONS:
|
| Method: | Indirect Enzyme Linked Immune Reaction |
| Capture: | C1q (human) |
| Calibrators: | 0, 6.3, 12.5, 25, 50, 100 U/ml
|
| Sample type: | Serum and plasma |
| Sample volume: | 10 µl |
| Measuring range: | 0 - 100 U/ml
|
| Reading: | Absorbance at 450 nm |
| Cut-off: | 25 U/ml |
| Total time: | 65 minutes
|
| Storage: | 2 - 8 ºC
|
| Kit Contents: | Microplate
Calibrators: A, B, C, D, E, F
Controls: Positive & Negative
Enzyme Conjugate
TMB Substrate
Diluent
Stop Solution
Wash Concentrate
|