| PRODUCT NAME: | Anti-dsDNA IgG |
| INTENDED USE: | Anti-dsDNA IgG is an ELISA test for the quantitative determination of IgG antibodies against double-stranded DNA in human serum or plasma. |
| PRODUCT OVERVIEW: | Antibodies to dsDNA are found during the active phases of SLE, where the serum concentration exhibits positive correlation to the severity of the disease. An ongoing therapy may be monitored by the aid of autoantibody determination. Diagnostic sensitivity of the anti-dsDNA determination in cases of SLE is approximately 91% combined with a diagnostic specificity of nearly 96%. Antibodies against DNA can be differentiated into two groups:
1. Antibodies, that bind only to native double-stranded DNA (dsDNA)
2. Antibodies recognizing single-stranded DNA (ssDNA) too. |
TECHNICAL SPECIFICATIONS:
|
| Method: | Indirect Enzyme Linked Immune Reaction |
| Capture: | dsDNA (recombinant) |
| Calibrators: | 0, 12.5, 25, 50, 100, 200 IU/ml |
| Calibrator traceability: | WHO Wo/80
|
| Sample type: | Serum |
| Sample volume: | 10 µl |
| Measuring range: | 0 - 200 U/ml
|
| Reading: | Absorbance at 450 nm |
| Cut-off: | 20 U/ml |
| Total time: | 65 minutes
|
| Storage: | 2 - 8 ºC
|
| Kit Contents: | Microplate
Calibrators: A, B, C, D, E, F
Controls: Positive and Negative
Sample Buffer
Enzyme conjugate
TMB Substrate
Wash solution
Stop Solution
|