INTRODUCTION:
Hemoglobin is a tetramer which is composed out of 2 α-chains and 2 non α-chains. In the normal synthesis, α-chains and non α-chains (β-chain, γ-chain and δ-chain) are synthesized with no overproduction or underproduction. Thalassemias are hereditary disorders that are caused by abnormally synthesized globin chains. In thalassemic disorders, due to a quantitative defect in synthesis of a specific chain, free α chains or single globin chains such as HbH(β4), HbBart’s(γ4) of hemoglobin could be synthesized. β-Thalassemia in an analogous manner means reduced or missing production of hemoglobin β-chains, which is compensated by overproduction of γ-chains and δ-chains. Consequently, hemoglobin A2 (α2δ2) and hemoglobin F(α2γ2) are often elevated in β-Thalassemia. The β-Thalassemia Analysis Mode of HLC-723G8 can separate HbF and HbA2 quickly and accurately. HbF and HbA2 are reported in a percentage of the total amount of hemoglobin present in a sample within 6.0 min.
CONSUMABLES:
TSKgel G8 β-Thal. HSi
The TSKgel G8 β-Thal. HSi is intended for in vitro diagnostic use for the measurement of Hemoglobin F and A2 in blood specimens. The TSKgel G8 β-Thal. HSi is designed exclusively for use with the Tosoh Automated Glycohemoglobin Analyzer HLC-723G8 which is based on High Performance Liquid Chromatography (HPLC).
G8 β-Thalassemia Elution Buffer Kit
G8 β-Thalassemia Elution Buffer Kit consist of Buffer No. 1 (S), Buffer No. 2 (S) and Buffer No. 3 (S). Buffer No. 1 (S). The three elution buffers contain different salt concentration and pH. A step gradient elution of these buffers is used to separate and assay HbF and HbA2.
HSi Hemolysis And Wash Solution
HSi Hemolysis And Wash Solution is designed exclusively for use with Tosoh Automated Glycohemoglobin Analyzer. It lysis and dilutes the whole blood specimen and then injects a small volume of this specimen onto the TSKgel G8 β-Thal. HSi column for measurement of stable-A1c.
G8 Hemoglobin F&A2 Calibrator
G8 Hemoglobin F&A2 Calibrator has been designed exclusively for the calibration of the Tosoh Automated Glycohemoglobin Analyzer HLC-723G8 β-Thalassemia Analysis Mode.
G8 Hemoglobin F&A2 Control Set
G8 Hemoglobin F&A2 Control Set has been designed exclusively for the quality control of Tosoh Automated Glycohemoglobin Analyzer HLC-723G8 β-Thalassemia Analysis Mode. These controls can be used to evaluate and monitor the precision of the performance of an assay in the same way as an unknown specimen. Two concentration levels are provided to cover the entire clinical range.
G8 β-Thalassemia Diluting Solution
G8 β-Thalassemia Diluting Solution is designed to manually dilute the samples when sample volume is too low or when haematocrit is too low where TOTAL AREA of the assay results may drop below 2000.